Biological Safety Cabinets


Biological safety cabinets (BSCs) are used to protect personnel, products and the environment from exposure to biohazards and cross contamination during routine procedures.

Classes of biological safety cabinets

Biological safety cabinets (BSCs) are used to protect personnel, products and the environment from exposure to biohazards and cross contamination during routine procedures. When choosing a biological safety cabinet for your work space, EH&S can help you select the class of cabinet that will provide the best protection.

There are three classes of BSCs: Class I, II and III. While all three classes afford personnel and environmental protection, only Class II and III cabinets provide product protection.

  • Class I BSCs are suitable for work involving low to moderate-risk agents. Since incoming air is not filtered, Class I BSCs should not be used with research materials (e.g., cell lines) that must be handled under sterile conditions. Class I cabinets are used to enclose equipment (e.g., centrifuges, harvesting equipment or small fermenters) or procedures with potential to generate aerosols (e.g., cage dumping, tissue homogenization or culture aeration). Because outgoing air is filtered, research personnel are protected while using a Class I BSC.
  • Class II BSCs provide a partial barrier for the safe manipulation of low, moderate and high-risk microorganisms. Class II cabinets, which are the most frequently used in research and clinical laboratories, are divided into four types (Types A1, A2, B1 and B2).
  • The Class III BSC is a totally enclosed, gas-tight ventilated cabinet, and provides the highest level of personnel, environmental and product protection. Operations within a Class III BSC are conducted through attached rubber gloves.

Selecting a biological safety cabinet

When purchasing a biological safety cabinet, you should consider the type of work that will be conducted in it.

Table 1 describes the principal characteristics of all classes and types of BSCs.









II / A1*




II / A2



No, unless thimble connected to the HVAC system, then low levels of volatile toxic chemicals allowed

II / B1



Yes: low levels of volatile toxic chemicals & trace radionuclides

II / B2



Yes: volatile toxic chemicals & radionuclides



N / A


Table 1: Characteristics of different classes and types of BSCs
*These cabinets are obsolete; no parts are available to repair the cabinet.

For more information, please see the Chemical Use in Biological Safety Cabinets Focus Sheet.

What you need to know

Contact EH&S if you plan to purchase or relocate a biological safety cabinet, or bring a unit to the UW from another university. EH&S can help you select the appropriate cabinet for your research, evaluate the space you are considering, and determine if necessary ventilation and utilities are available.

Your cabinet needs to be tested and recertified annually by a qualified EH&S safety professional. If EH&S has your cabinet in its inventory, we will contact you about a week in advance of our service.

If your cabinet sounds an alarm or does not appear to be functioning as intended, stop your work and contact EH&S for assistance.


Services available

EH&S provides the following services:

  • Performs certification service for biological safety cabinets to verify and assure proper performance; BSCs are tested to manufacturer's specifications and to National Sanitation Foundation Standard 49.
  • Maintains design standards for new construction and provides consultation on cabinet selection, performance, decontamination, relocation and design.

Frequently Asked Questions (FAQ)

For information on replacing obsolete biosafety cabinets, please refer to the following resources:

Review products available from the companies outlined in the attached list of approved BSCs and complete the online Biological Safety Cabinet Purchase-Move Request form.

For additional information, please read the Steps to Purchase, Replace, Relocate or Remove a Biosafety Cabinet.

EH&S does not prohibit UV light and you may choose to include a UV light when ordering a BSC. However, it is our opinion that is not necessary nor recommended in biosafety cabinets. The EH&S annual certification of the cabinet does NOT include a verification of the efficacy of the UV light.

For more information, see "Position Paper on the Use of Ultraviolet Lights in Biological Safety Cabinets" (Burgener, July, 2006, Applied Biosafety, 11(4) pp. 228-230) and read the article "The trouble with UV light in your biosafety cabinet."

Design standards may be found in the EH&S lab safety design guide.

Refer to the list of safety professionals according to your location.

EH&S regularly reviews the fees for services we provide to users of biological safety cabinets (BSCs) to ensure our fees match the expenses we incur for these services.

The table below shows rates for BSC services provided by EH&S that are in effect as of July 1, 2019. 



 BSC Certification Test   $323
 BSC Airflows Only   $98 
 BSC Decon for Relocation   $294
 BSC Decon for Motor Change   $294
 BSC Decon for Filter Change   $0
 BSC Filter Change and Retest   $0
 BIBO Decon-Certek, Filter Scan   $392
 BIBO Decon-No Certek, Filter Scan   $147
 BIBO Filter Change, Filter Scan   $0
 Other service (per hour)  $49

Laboratories can experience ventilation shutdowns. These shutdowns can be the result of planned maintenance work as well as unplanned events such as equipment failure or power outages.

Do not use biological safety cabinets (BSCs) with canopies when the ventilation system serving a room is not functioning. This applies to both supply air as well as exhaust air. Inadequate ventilation associated directly with your equipment or indirectly with your room can compromise personal protection. The product protection provided by a BSC can also be compromised.

If you have concerns regarding your biological safety cabinet during a shutdown or need help troubleshooting issues with your equipment, contact Environmental Health & Safety at 206.543.9510.